Amplification plots for IFNG using a kit from Supplier S.| Q-Bond in Rotor-Gene Multiplex PCR Master Mix increases the affinity of DNA polymerase for short single-stranded DNA, reducing primer annealing time to a few seconds.
Amplification plots for IFNG using the Rotor-Gene Multiplex PCR Kit. Amplification plots for TNF using a kit from Supplier S. Amplification plots for TNF using the Rotor-Gene Multiplex PCR Kit. Lower C T values on the Rotor-Gene Q demonstrate detection with greater sensitivity. C T values obtained for all 4 targets (instrument and kit from Supplier S did not successfully detect IFNG N.D.). Targets amplified, and reporter dyes of corresponding TaqMan probes. Reactions were run in triplicate using either the Rotor-Gene Q and Rotor-Gene Multiplex PCR Kit or an instrument and kit from Supplier S. The C T values were comparable with those achieved in control singleplex reactions, demonstrating the reliability of the duplex assay.|Tenfold dilutions of human leukocyte cDNA (10 ng to 10 pg) were used as template in 4-plex, real-time PCR. Analysis of tenfold dilutions of leukocyte cDNA template from 100 ng to 1 pg provided high PCR efficiencies of around 95%. Commercial Partner and Distributor Solutionsĭuplex, real-time two-step RT-PCR was carried out on the Rotor-Gene Q using the Rotor-Gene Multiplex PCR Kit and self-designed TaqMan assays for IL8 (interleukin 8) and ACTB (β-actin).
Solutions for Laboratory-Developed Tests.Whole Genome/Transcriptome Amplification.SYBR Green- or Dye-Based One-Step qRT-PCR.Reverse Transcription & cDNA Synthesis for qPCR.Tagged Protein Expression, Purification, Detection.
0 kommentar(er)
